Imikhiqizo
Izimpawu Zenhliziyo - Troponin I
I-Troponin iyiphrotheni elawulayo emicu yemisipha emangqamuzaneni emisipha, elawula ngokuyinhloko ukushelela okuhlobene phakathi kwemisipha emincane emincane emincane phakathi nokufinyela kwe-myocardial.Iqukethe ama-subunits amathathu: i-troponin T (TNT), i-troponin I (TNI) ne-troponin C (TNC).Ukuvezwa kwezinhlobo ezintathu ze-subtypes ku-skeletal muscle kanye ne-myocardium nakho kulawulwa yizakhi zofuzo ezahlukene.Okuqukethwe kwe-troponin yenhliziyo ku-serum evamile kuphansi kakhulu kunamanye ama-enzyme e-myocardial, kodwa ukuhlushwa kwe-cardiomyocytes kuphezulu kakhulu.Lapho ulwelwesi lweseli lwe-myocardial lungaguquki, i-cTnI ayikwazi ukungena kulwelwesi lweseli ekujikelezeni kwegazi.Lapho amangqamuzana e-myocardial ewohloka kanye ne-necrosis ngenxa ye-ischemia ne-hypoxia, i-cTnI idedelwa egazini ngolwelwesi lwamaseli olimele.Ukuhlushwa kwe-cTnI kuqala ukukhuphuka emahoreni angama-3-4 ngemuva kokuvela kwe-AMI, kukhuphuke ngamahora ayi-12-24, futhi kuqhubeke izinsuku ezingama-5-10.Ngakho-ke, ukuzimisela kokugxila kwe-cTnI egazini kuye kwaba inkomba enhle yokubhekwa kokuphumelela kokuphindaphinda nokuphindaphinda kweziguli ze-AMI.I-cTnI ayinakho ukucaciswa okuqinile kuphela, kodwa futhi inokuzwela okuphezulu kanye nobude besikhathi eside.Ngakho-ke, i-cTnI ingasetshenziswa njengomaka obalulekile ukusiza ekuxilongweni kokulimala kwe-myocardial, ikakhulukazi i-acute myocardial infarction.
Izingxenye Eziyinhloko
| Izinhlayiya ezincane (M): | 0.13mg/ml Ama-Microparticles ahlanganiswe ne-anti troponin I ultra antibody |
| I-Reagent 1 (R1): | 0.1M Tris buffer |
| I-Reagent 2 (R2): | 0.5μg/ml I-Alkaline phosphatase ebhalwe ukuthi i-antitroponin I ultra |
| Isixazululo sokuhlanza: | 0.05% surfactant,0.9% Sodium chloride buffer |
| I-Substrate: | I-AMPPD kubhafa ye-AMP |
| I-Calibrator (uyazikhethela): | I-Troponin I ultra antigen |
| Izinto zokulawula (ongakukhetha): | I-Troponin I ultra antigen |
Qaphela:
1.Izingxenye azishintsheki phakathi kwamaqoqo ama-reagent strips;
2.Bheka ilebula yebhodlela le-calibrator ukuze uthole ukugxila kwe-calibrator;
3.Bona ilebula yebhodlela lokulawula ukuze uthole uhla lokuhlushwa lwezilawuli.
Isitoreji Nokufaneleka
1.Isitoreji: 2℃～8℃, gwema ukukhanya kwelanga okuqondile.
2.Ukuqinisekisa: imikhiqizo engavuliwe isebenza izinyanga eziyi-12 ngaphansi kwezimo ezithile.
3.Ama-Calibrator nezilawuli ngemva kokuncibilika kungagcinwa izinsuku eziyi-14 endaweni engu-2℃～8℃ emnyama.
Izinsimbi Ezisebenzayo
Isistimu ye-CLIA ezenzakalelayo ye-Illumaxbio (lumiflx16, lumiflx16s, lumilite8, lumilite8s).
