Imikhiqizo
Izimpawu Zenhliziyo - hs-cTnI
I-High-Sensitivity Troponin I Assays
Imininingwane
Imichilo engama-24/ibhokisi, imichilo engama-48/ibhokisi
Isimiso Sokuhlola
Umgomo wesandwich we-Microparticle chemiluminescence immunoassay.
Engeza isampula, isivikelo sokuhlaziya, ama-microparticles ambozwe nge-troponin I ultra antibody, i-alkaline phosphatase ebhalwe ukuthi i-troponin I ultra antibody eshubhuni yokusabela ukuze uthole ukusabela okuxubile.Ngemva kokufakwa ekufukameleni, amasayithi ahlukene e-troponin I ultra antigen kusampula abophezela ku-troponin I-ultra antibody kubuhlalu kazibuthe kanye ne-troponin I ultra antibody kumaka we-alkaline phosphatase ngokulandelana ukuze kwakhe i-enzyme ye-antibody yesigaba esiqinile ebizwa ngokuthi inkimbinkimbi ye-antibody.Izinto eziboshelwe kubuhlalu kazibuthe zikhangiswa amandla kazibuthe, kuyilapho i-enzyme engaboshiwe ebizwa ngokuthi amasosha omzimba nezinye izinto zikhukhulwa.Bese ixutshwa ne-chemiluminescent substrate.I-luminescent substrate ikhipha ama-photon ngaphansi kwesenzo se-alkaline phosphatase.Inani lama-photons akhiqizwayo lilingana ngokuqondile nokugxiliswa kwe-troponin I ultra kusampula.Ngokusebenzisa ijika lokulinganisa lobuningi be-concentration-photon, ukugxiliswa kwe-cTnI kusampula kungabalwa.
Izingxenye Eziyinhloko
| Izinhlayiya ezincane (M): | 0.13mg/ml Ama-Microparticles ahlanganiswe ne-anti troponin I ultra antibody |
| I-Reagent 1 (R1): | 0.1M Tris buffer |
| I-Reagent 2 (R2): | 0.5μg/ml I-Alkaline phosphatase ebhalwe ukuthi i-anti troponin I ultra antibody |
| Isixazululo sokuhlanza: | 0.05% surfactant,0.9% Sodium chloride buffer |
| I-Substrate: | I-AMPPD kubhafa ye-AMP |
| I-Calibrator (uyazikhethela): | troponin I ultra antigen |
| Izinto zokulawula (ongakukhetha): | troponin I ultra antigen |
Qaphela:
1.Izingxenye azishintsheki phakathi kwamaqoqo ama-reagent strips;
2.Bheka ilebula yebhodlela le-calibrator ukuze uthole ukugxila kwe-calibrator;
3.Bona ilebula yebhodlela lokulawula ukuze uthole uhla lokuhlushwa lwezilawuli;
Isitoreji Nokufaneleka
1.Isitoreji: 2℃～8℃, gwema ukukhanya kwelanga okuqondile.
2.Ukuqinisekisa: imikhiqizo engavuliwe isebenza izinyanga eziyi-12 ngaphansi kwezimo ezithile.
3.Ama-Calibrator nezilawuli ngemva kokuncibilika kungagcinwa izinsuku eziyi-14 endaweni engu-2℃～8℃ emnyama.
Ithuluzi Elisebenzayo
I-Automated CLEIA System ye-Illumaxbio(lumiflx16、lumiflx16s、lumilite8、lumilite8s).
